User profiles for A. W. Cheng
Albert W. Cheng, PhD 郑瑚博士Professor at Institute of Zoology, Chinese Academy of Sciences Verified email at cheng.bio Cited by 22267 |
Histone H3K27ac separates active from poised enhancers and predicts developmental state
MP Creyghton, AW Cheng… - Proceedings of the …, 2010 - National Acad Sciences
Developmental programs are controlled by transcription factors and chromatin regulators,
which maintain specific gene expression programs through epigenetic modification of the …
which maintain specific gene expression programs through epigenetic modification of the …
[HTML][HTML] One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering
…, H Yang, CS Shivalila, MM Dawlaty, AW Cheng… - cell, 2013 - cell.com
Mice carrying mutations in multiple genes are traditionally generated by sequential
recombination in embryonic stem cells and/or time-consuming intercrossing of mice with a single …
recombination in embryonic stem cells and/or time-consuming intercrossing of mice with a single …
[HTML][HTML] One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering
The type II bacterial CRISPR/Cas system is a novel genome-engineering technology with the
ease of multiplexed gene targeting. Here, we created reporter and conditional mutant mice …
ease of multiplexed gene targeting. Here, we created reporter and conditional mutant mice …
Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells
Bacterial type II CRISPR-Cas9 systems have been widely adapted for RNA-guided genome
editing and transcription regulation in eukaryotic cells, yet their in vivo target specificity is …
editing and transcription regulation in eukaryotic cells, yet their in vivo target specificity is …
Human embryonic stem cells with biological and epigenetic characteristics similar to those of mouse ESCs
Human and mouse embryonic stem cells (ESCs) are derived from blastocyst-stage embryos
but have very different biological properties, and molecular analyses suggest that the …
but have very different biological properties, and molecular analyses suggest that the …
[HTML][HTML] Single-cell expression analyses during cellular reprogramming reveal an early stochastic and a late hierarchic phase
During cellular reprogramming, only a small fraction of cells become induced pluripotent
stem cells (iPSCs). Previous analyses of gene expression during reprogramming were based …
stem cells (iPSCs). Previous analyses of gene expression during reprogramming were based …
[HTML][HTML] Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system
Technologies allowing for specific regulation of endogenous genes are valuable for the
study of gene functions and have great potential in therapeutics. We created the CRISPR-on …
study of gene functions and have great potential in therapeutics. We created the CRISPR-on …
[HTML][HTML] Generation of isogenic pluripotent stem cells differing exclusively at two early onset Parkinson point mutations
F Soldner, J Laganière, AW Cheng, D Hockemeyer… - Cell, 2011 - cell.com
Patient-specific induced pluripotent stem cells (iPSCs) derived from somatic cells provide a
unique tool for the study of human disease, as well as a promising source for cell …
unique tool for the study of human disease, as well as a promising source for cell …
[PDF][PDF] Tet1 is dispensable for maintaining pluripotency and its loss is compatible with embryonic and postnatal development
The Tet family of enzymes (Tet1/2/3) converts 5-methylcytosine (5mC) to 5-hydroxymethylcytosine
(5hmC). Mouse embryonic stem cells (mESCs) highly express Tet1 and have an …
(5hmC). Mouse embryonic stem cells (mESCs) highly express Tet1 and have an …
[HTML][HTML] An EMT–driven alternative splicing program occurs in human breast cancer and modulates cellular phenotype
Epithelial-mesenchymal transition (EMT), a mechanism important for embryonic development,
plays a critical role during malignant transformation. While much is known about …
plays a critical role during malignant transformation. While much is known about …