User profiles for A. W. Cheng

Albert W. Cheng, PhD 郑瑚博士

Professor at Institute of Zoology, Chinese Academy of Sciences
Verified email at cheng.bio
Cited by 22267

Histone H3K27ac separates active from poised enhancers and predicts developmental state

MP Creyghton, AW Cheng… - Proceedings of the …, 2010 - National Acad Sciences
Developmental programs are controlled by transcription factors and chromatin regulators,
which maintain specific gene expression programs through epigenetic modification of the …

[HTML][HTML] One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering

…, H Yang, CS Shivalila, MM Dawlaty, AW Cheng… - cell, 2013 - cell.com
Mice carrying mutations in multiple genes are traditionally generated by sequential
recombination in embryonic stem cells and/or time-consuming intercrossing of mice with a single …

[HTML][HTML] One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering

H Yang, H Wang, CS Shivalila, AW Cheng, L Shi… - Cell, 2013 - cell.com
The type II bacterial CRISPR/Cas system is a novel genome-engineering technology with the
ease of multiplexed gene targeting. Here, we created reporter and conditional mutant mice …

Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells

…, AC Chiu, PD Hsu, DB Dadon, AW Cheng… - Nature …, 2014 - nature.com
Bacterial type II CRISPR-Cas9 systems have been widely adapted for RNA-guided genome
editing and transcription regulation in eukaryotic cells, yet their in vivo target specificity is …

Human embryonic stem cells with biological and epigenetic characteristics similar to those of mouse ESCs

J Hanna, AW Cheng, K Saha, J Kim… - Proceedings of the …, 2010 - National Acad Sciences
Human and mouse embryonic stem cells (ESCs) are derived from blastocyst-stage embryos
but have very different biological properties, and molecular analyses suggest that the …

[HTML][HTML] Single-cell expression analyses during cellular reprogramming reveal an early stochastic and a late hierarchic phase

Y Buganim, DA Faddah, AW Cheng, E Itskovich… - Cell, 2012 - cell.com
During cellular reprogramming, only a small fraction of cells become induced pluripotent
stem cells (iPSCs). Previous analyses of gene expression during reprogramming were based …

[HTML][HTML] Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system

AW Cheng, H Wang, H Yang, L Shi, Y Katz… - Cell research, 2013 - nature.com
Technologies allowing for specific regulation of endogenous genes are valuable for the
study of gene functions and have great potential in therapeutics. We created the CRISPR-on …

[HTML][HTML] Generation of isogenic pluripotent stem cells differing exclusively at two early onset Parkinson point mutations

F Soldner, J Laganière, AW Cheng, D Hockemeyer… - Cell, 2011 - cell.com
Patient-specific induced pluripotent stem cells (iPSCs) derived from somatic cells provide a
unique tool for the study of human disease, as well as a promising source for cell …

[PDF][PDF] Tet1 is dispensable for maintaining pluripotency and its loss is compatible with embryonic and postnatal development

…, K Ganz, BE Powell, YC Hu, S Markoulaki, AW Cheng… - Cell stem cell, 2011 - cell.com
The Tet family of enzymes (Tet1/2/3) converts 5-methylcytosine (5mC) to 5-hydroxymethylcytosine
(5hmC). Mouse embryonic stem cells (mESCs) highly express Tet1 and have an …

[HTML][HTML] An EMT–driven alternative splicing program occurs in human breast cancer and modulates cellular phenotype

IM Shapiro, AW Cheng, NC Flytzanis, M Balsamo… - PLoS …, 2011 - journals.plos.org
Epithelial-mesenchymal transition (EMT), a mechanism important for embryonic development,
plays a critical role during malignant transformation. While much is known about …