Toxoplasmosis and pregnancy

Effect on pregnancy

Infection with T gondii before pregnancy confers little or no risk to the fetus except in women who become infected up to 3 months before conception.^9,10 In the neonate, manifestations of congenital toxoplasmosis might include hydrocephalus, microcephaly, intracranial calcifications, retinochoroiditis, strabismus, blindness, epilepsy, psychomotor and mental retardation, petechiae due to thrombocytopenia, and anemia.^11,12

While infection in early pregnancy poses a small risk of fetal transmission (less than 6%), rates of transmission range between 60% and 81% in the third trimester.¹³ Conversely, although the transmission of T gondii during embryogenesis is rare, it results in far more serious effects on the fetus.¹⁴ In contrast, maternal infection in the third trimester often results in asymptomatic newborns. However, if not treated appropriately, these newborns might develop retinochoroiditis and neurologic deficits in childhood or early adulthood.^14–16

There is no evidence of T gondii transmission through breastfeeding or via direct human-to-human contact.³

Diagnosis

The greatest challenge in diagnosing toxoplasmosis is to establish the acute (primary) infection and distinguish it from past (chronic) infection. Toxoplasma gondii infection can be diagnosed using serologic tests, ultrasound scans, and amniocentesis. Results of serologic tests measuring immunoglobulin (Ig) M and IgG are often difficult to interpret when differentiating between acute and chronic infections. Following acute infection, IgM antibody titres rise starting on day 5 and reach the maximum level at 1 to 2 months. At this point, IgM antibodies decline more rapidly than IgG antibodies.¹⁷ However, in many cases the IgM antibodies persist for years following acute infection. In contrast, IgG antibodies are usually detectable within 1 to 2 weeks after acute infection, peak within 12 weeks to 6 months, and usually remain detectable throughout life.¹⁸ The absence of IgG and IgM antibodies before or early in pregnancy indicates no previous infection and identifies women at risk of acquiring the infection during pregnancy.¹⁹ The detection of IgG antibodies and absence of IgM antibodies indicates an old infection. However, if test results are positive for both IgG and IgM, interpretation is difficult, as the positive results might be owing to either a recent infection or low levels of IgM antibodies from a previous infection.²⁰ If acute infection is suspected, repeat testing is recommended within 2 to 3 weeks.¹⁷ A 4-fold rise in IgG antibody titre between tests indicates a recent infection.²¹

Confirming primary infection is of utmost importance in evaluating the risk of fetal transmission, initiating antibiotics, and providing appropriate counseling. To more accurately determine the likelihood of a recently acquired infection, more specific tests, such as IgG antibody avidity testing, are helpful.²² The IgG avidity assay measures the strength of IgG binding to T gondii.²¹ In most cases IgG avidity shifts from a low to a high index about 5 months after the infection. Thus, patients with acute infection exhibit a low avidity index, suggesting that infection occurred within 5 months of testing, whereas those with previous infection have a high IgG avidity index.²³

Up to two-thirds of cases of congenital toxoplasmosis do not exhibit any abnormality on ultrasound scans.²⁴ Positive sonographic findings for T gondii infection include intracranial calcifications, microcephaly, hydrocephalus, ventricular dilations, hepatosplenomegaly, ascites, and severe intrauterine growth retardation.³

As maternal infection does not always result in fetal infection, it is critical to determine whether fetal infection has occurred. The diagnosis of congenital toxoplasmosis in the fetus is currently done by polymerase chain reaction analysis of the amniotic fluid.⁶ Amniocentesis is offered only if maternal primary infection is confirmed, if maternal serologic test results do not confirm or exclude acute infection, or if ultrasonographic features are consistent with congenital toxoplasmosis.²⁵ It should be offered after 18 weeks’ gestation and at least 4 weeks after the suspected acute maternal infection to decrease the risk of false-negative results.²⁵

Management

While there is insufficient evidence to prove that treating mothers with seroconversion during pregnancy prevents fetal infection, treatment might reduce the severity of congenital toxoplasmosis.^26,27 If primary T gondii infection is confirmed during pregnancy, treatment is used for fetal prophylaxis or to decrease the disease severity.^28,29 In case of maternal infection without fetal infection, spiramycin is the drug of choice to prevent vertical transmission.²⁸ Spiramycin is a macrolide antibiotic that cannot cross the placenta but remains concentrated in it. According to the Society of Obstetricians and Gynaecologists of Canada guidelines, it is prescribed at a dosage of 1 g orally every 8 hours for the duration of pregnancy if amniotic fluid polymerase chain reaction analysis results are negative for T gondii.²⁵

Pyrimethamine and sulfadiazine are administered in cases of confirmed fetal infection, but not in cases of suspected infection, especially in the first trimester, owing to potential teratogenicity and bone marrow toxicity to both mother and fetus.^26,28 Both prenatal and postnatal treatment have shown evidence of reducing the risk and severity of long-term symptoms. However, even after treatment has been discontinued, clinical and ophthalmologic examinations should be performed regularly for several years to screen for any sequelae that might arise.⁶

Prevention

In Canada, owing to the low prevalence of the disease and the aforementioned limitations in diagnosis and therapy, routine screening for toxoplasmosis is currently not recommended in low-risk populations.²⁵ Only Nunavik and other parts of northern Quebec have screening programs for T gondii during pregnancy owing to high endemic seroprevalence.³⁰ In many European countries, universal screening for T gondii is provided but benefit and costs have not been adequately assessed.¹³ Routine screening is not recommended in most countries where incidence of toxoplasmosis is low, including in the United Kingdom and United States.²⁷

Prevention of congenital toxoplasmosis is dependent on effective avoidance of infection during pregnancy.³¹ This involves avoidance of contact with litter boxes or soil, owing to the presence of T gondii oocytes that might have been excreted by cats. Pet cats are less likely to be a source of infection if they are kept indoors only and are provided with only cooked, preserved, or dry food.^32,33

It is also important to avoid undercooked, raw, or cured meat, and raw, unwashed fruits or vegetables.³¹ Proper hand hygiene is essential to decreasing the risk of infection.⁶

Counseling

Owing to the grave outcome of fetal toxoplasmosis, it is critical to counsel the parents and ensure that they understand the implications of positive and negative test results.